ALEXANDER ROUVINSKI,FELIX AUGUSTO REY,GAVIN SCREATON,GIOVANNA BARBA-SPAETH,JUTHATHIP MONGKOLSAPAYA,MARIE-CHRISTINE VANEY,PABLO GUARDADO-CALVO,STÉPHANE DUQUERROY
申请号：
BR112017001340
公开号：
BR112017001340A2
申请日:
2015.07.23
申请国别(地区):
BR
年份:
2017
代理人:
摘要：
It is a dengue virus envelope dimer (ede) epitope, wherein ede: a) encompasses the polypeptides of a dengue virus envelope polypeptide dimer； and / or b) is presented in an envelope protein dimer； and / or c) is formed from consecutive or non-consecutive residues of the envelope polypeptide dimer, wherein the dimer is a homodimer or heterodimer of native and / or mutant envelope polypeptides of any one or two of denv-1. , denv-2, denv-3 and denv-4. the ede may be a stabilized recombinant dengue virus envelope and glycoprotein ectodomain (s) dimer, wherein the dimer is: covalently stabilized with at least one disulfide interlinkage bond between the two monomers of se, and / or covalently stabilized. with at least one sulfhydryl reactive crosslinker between the two se monomers, and / or covalently stabilized by bonding the two se monomers via modified sugars； and / or covalently stabilized by being formed as a single polypeptide chain, optionally with a linker region, optionally a serine glycine rich linker region, which separates the sequences from and / or non-covalently stabilized by substitution of at least one. amino acid residue in the amino acid sequence of at least one monomer and at least one bulky side chain amino acid at the dimer interface or domain 1 (d1) / domain 3 (d3) linker of each monomer. a compound, for example, an antibody or antibody fragment that can neutralize more than one dengue virus serotype, for example, an antibody that can bind to an ede of the invention.trata-se de um epítopo de dímero de envelope (ede) de vírus da dengue, em que o ede: a) abrange os polipeptídeos de um dímero de polipeptídeo de envelope de vírus da dengue； e/ou b) é apresentado em um dímero de proteínas envelope； e/ou c) é formado a partir de resíduos consecutivos ou não consecutivos do dímero de polipeptídeo de envelope, em que o dímero é um homodímero ou heterodímero de polipeptídeos de envelope nativos e/ou mutantes, de qualquer um ou dois dentre denv-1, denv-2, den
