ABSTRACT Exemplary embodiments of the present disclosure are directed towards a wound scratch imaging and monitoring device comprising of: a wounding template with a plurality of insertion slots configured to fit into the experimental multi-well plate with a guide positioned at the centre a wounding device configured to have an array of wounding probes accommodated by an armature, whereby each of the wounding probe is configured to penetrate the monolayer of the plurality of penetrable cells using a probing tip an array of illuminating device positioned to flash the light rays on the plurality of penetrable cells an array of imaging device configured to capture the images of the plurality of penetrable cells, whereby the plurality of penetrable cells is the imaging target an array of ultrasound emission device configured to emit the ultrasound pulse by emission of acoustic waves upon the infliction of wound and an array of ultrasound receiving device configured to receive the emitted acoustic waves upon the infliction of wound, whereby ultrasound receiving device sends out the pulse and integrates the pulse into the display device.