A physiological signal sensing system is disclosed, which includes a physiological signal sensing device and a mobile device. The physiological signal sensing device is used to sense the physiological status of a user for obtaining a physiological signal and to generate a command according to the physiological signal. The mobile device is used to receive the command and generate a voice signal according to the command and a multiple of pre-built language information, and then outputs the voice signal. A physiological signal sensing method is also disclosed.一種生理訊號感測系統,包含一生理訊號感測裝置,及一行動裝置。該生理訊號感測裝置用以感測使用者的生理狀態以獲得一生理訊號,並根據該生理訊號產生一指令。該行動裝置用以接收該指令,並根據該指令與多數筆預先建立的語言資料產生一語音訊號,繼而輸出該語音訊號。一種生理訊號感測方法亦被揭露。1‧‧‧生理訊號感測系統2‧‧‧生理訊號感測裝置21‧‧‧感測單元22‧‧‧處理單元23‧‧‧傳輸單元3‧‧‧行動裝置31‧‧‧收發模組32‧‧‧分析模組33‧‧‧記憶模組34‧‧‧語音處理模組35‧‧‧輸出模組4‧‧‧伺服器裝置41‧‧‧傳送單元42‧‧‧儲存模組