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METHODS AND COMPOSITIONS FOR MARKER-FREE GENOME MODIFICATION
专利权人:
发明人:
ANDREW MARK CIGAN,SERGEI SVITASHEV
申请号:
US15763758
公开号:
US20180273960A1
申请日:
2016.10.17
申请国别(地区):
US
年份:
2018
代理人:
摘要:
Compositions and methods are provided for modifying a nucleotide sequence in the genome of a plant cell, without the use of a selectable marker. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to make a double strand break in a target site located in a nucleotide sequence and plant cells are obtained without the use of a selectable marker, and to provide an effective system for modifying target sites within the genome of a plant, plant cell or seed. Compositions and methods are also provided for producing a plant cell, callus tissue or plant having a modified nucleotide sequence in its genome, without the use of a selectable marker.
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