PROCÉDÉ DE FABRICATION D'UN ANTICORPS VH CHAÎNE LOURDE DANS UN MAMMIFÈRE TRANSGÉNIQUE ET COMPLEXE DE LIAISON COMPRENANT UN DIMÈRE D'UNE PREMIÈRE CHAÎNE ET D'UNE SECONDE CHAÎNE DANS LEQUEL CHAQUE CHAÎNE EST CONSTITUÉE DE DEUX DOMAINES DE LIAISON VH SPÉCIFIQUES D'UN ANTIGÈNE LIÉS PAR UN DOMAINE DE DIMÉRISATION
Erasmus University Medical Center Rotterdam;Craig, Roger Kingdon
发明人:
申请号：
EP17173113.6
公开号：
EP3272770A1
申请日:
2005.07.22
申请国别(地区):
EP
年份:
2018
代理人:
摘要：
The present invention relates to the manufacture of a diverse repertoire of functional heavy chain-only antibodies that undergo affinity maturation, and uses thereof. The invention also relates to the manufacture and use of a diverse repertoire of class-specific heavy chain-only antibodies and to the manufacture and use of multivalent polypeptide complexes with antibody heavy chain functionality, preferably antibody heavy chain binding functionality, constant region effector activity and, optionally, additional effector functions.The present invention also relates to a method of generation of fully functional heavy chain-only antibodies in transgenic mice in response to antigen challenge. In particular, the present invention relates to a method for the generation of human antigen-specific, high affinity, heavy chain-only antibodies of any class, or mixture of classes and the isolation and expression of fully functional VH antigen-binding domains.
