The invention relates to a dendritic cell tumor vaccine. The dendritic cell tumor vaccine is made by co-culturing dendritic cell and cancer stem cell. The preparation method of the dendritic cell tumor vaccine comprises the following steps: providing a cancer stem cell derived from tumor tissues, screening the cancer stem cell by a flow cytometry, radiating the cancer stem cell and co-culturing the cancer stem cell with the dendritic cell to obtain the dendritic cell tumor vaccine.本發明提供一種樹突狀細胞腫瘤疫苗,其係為樹突狀細胞和癌幹細胞共同培養而得。本發明另亦提供一種樹突狀細胞腫瘤疫苗的製備方法,係自腫瘤組織中分離癌幹細胞,並經由流式細胞儀篩選出癌幹細胞,以放射線照射目標癌幹細胞後,與樹突狀細胞共同培養,以得樹突狀細胞腫瘤疫苗。
