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Transgenic bird expressing foreign gene using endoplasmic reticulum chaperone promoter
专利权人:
Kenji Kyogoku
发明人:
Kenji Kyogoku,Hiroyuki Watanabe
申请号:
US14342208
公开号:
US09510571B2
申请日:
2012.08.27
申请国别(地区):
US
年份:
2016
代理人:
摘要:
An aim is to produce a suitable foreign protein in egg white in transgenic birds at levels equivalent to or higher than the expression levels achieved using an ovalbumin promoter or an actin promoter, and to reduce the great burden on birds by reducing the expression at sites other than the oviduct while achieving expression sufficient to predict the expression levels before the birds reach sexual maturity. Provided is a transgenic bird containing a nucleic acid base sequence in chromosome in a cell that forms the oviduct, the sequence containing: (a) an avian endoplasmic reticulum chaperone promoter and (b) a nucleic acid base sequence encoding a suitable foreign protein, functionally linked to the promoter. Also provided is a method for producing a suitable foreign protein, including recovering the suitable foreign protein from the transgenic bird. Further provided is a method for producing a transgenic bird, including introducing a nucleic acid base sequence into a chromosome in a cell that forms the oviduct, the sequence containing: (a) an avian endoplasmic reticulum chaperone promoter and (b) a nucleic acid base sequence encoding a suitable foreign protein, functionally linked to the promoter.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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