The present invention relates to a three-dimensional acupuncture point mapping method. The three-dimensional acupuncture point mapping method includes the steps of: (a) using a three-dimensional standard image including at least one part of a human body to generate a standard template for determining the standard acupuncture point positions; (b) determining the standard acupuncture point positions with respect to the three-dimensional standard image based on the standard template as well as mapping the determined standard acupuncture point positions in the three-dimensional standard image as three-dimensional coordinates of the acupuncture points; (c) displaying three-dimensional and in-vivo acupuncture point information generated by mapping the three-dimensional acupuncture point positions in the three-dimensional standard image; (d) identifying anatomical structures in a three-dimensional image based on the standard template upon obtaining the three-dimensional image of a user; (e) comparing the three-dimensional image with the three-dimensional standard image to calculate the difference between the anatomical structures in the three-dimensional image and the three-dimensional standard image; and (f) correcting the standard positions based on the calculated difference in the anatomical structures.本發明涉及三維經穴映射方法,包括如下步驟:(a)利用包括人體至少一部分部位的三維標準圖像生成標準範本;(b)基於標準範本確定經穴標準位置,將確定的標準位置映射到三維標準圖像;(c)顯示由標準位置映射到三維標準圖像而生成的三維體內經穴資訊;(d)獲得使用者的三維圖像後,基於標準範本識別三維圖像中的解剖結構;(e)通過比較三維圖像與三維標準圖像,以三維標準圖像中的解剖結構為准計算三維圖像和三維標準圖像中解剖結構的差異;(f)基於計算差異補正標準位置;及(g)考慮用戶輸入的施術者刺針熟練度及患者疾病類型補正刺針資訊。