The present invention provides a tubular tissue preparation device provided with: a chamber for accommodating a tubular support body； a first support part for communicating the inside and the outside of the chamber and fixing the tubular support body； and a pressurizing/depressurizing means for controlling the pressure in an inner space of the chamber, wherein the first support part is for fixing a first opening section of the tubular support body, communicating a lumen of the tubular support body and the outside of the chamber, and separating the lumen of the tubular support body and the inner space of the chamber. Also, the present invention provides a tubular tissue preparation kit that includes said device and a device for transporting sheet-shaped tissue. In addition, the present invention provides a tubular tissue preparation method using said device.
