Provided are a method for constructing a mouse model with conditional knockout of the Tmem30a gene from a pancreatic β cell and the use. The construction method comprises the steps of: constructing a homozygote mouse with conditional knockout of the Tmem30a gene, wherein both ends of one or more exons of the Tmem30a gene are inserted into directly arrayed loxP loci; and mating the mouse with a pancreatic β cell specific transgenic mouse Ins2-Cre, thereby obtaining the mouse model with conditional knockout of the Tmem30a gene from the pancreatic β cell. The mouse with conditional knockout of the Tmem30a gene from the pancreatic β cell shows glucose intolerance and poor insulin sensitivity, and can be used as a diabetes research model.
