The present invention relates to a pen-type medical fluorescent imaging device comprising: a probe which is elongated in the longitudinal direction and has an image capture unit at one end a plurality of light source units surrounding the image capture unit and a control unit for controlling the light source units, wherein the light source units comprise: a first light source for emitting light of a first wavelength range so that a blood vessel is marked by a first fluorescent material and a second light source for emitting light of a second wavelength range so that a glioma is marked by a second fluorescent material, wherein the first and second light sources are selectively controlled by the control unit.