One aspect of the invention provides a phantom including a confined fluidic path defining a plurality of regions of monotonically decreasing, discrete cross-sectional dimensions with respect to an imaging plane. Another aspect of the invention provides a method of assessing imaging. The method includes: placing a phantom as described herein within an imaging system； flowing one or more fluids through the phantom； and capturing one or more images of the phantom. Another aspect of the invention provides a kit for assessing imaging. The kit includes a phantom as described herein and instructions for use.
