PROBLEM TO BE SOLVED: To provide a means for efficiently differentiating, inducing and amplifying hematopoietic stem cells having long-term bone marrow reconstruction ability from pluripotent stem cells, particularly human pluripotent stem cells.SOLUTION: There are provided: a differentiation inducing promoter from pluripotent stem cells to hematopoietic stem cells and/or hematopoietic precursor cells the differentiation inducing promoter comprising a transforming growth factor-&beta (TGF-&beta) inhibitor such as one represented by the following formula (1) a composition containing the TGF-&beta inhibitor for inducing differentiation from pluripotent stem cells to hematopoietic stem cells and/or hematopoietic precursor cells a reagent kit containing the TGF-&beta inhibitor as the reagent for inducing differentiation from pluripotent stem cells to hematopoietic stem cells and/or hematopoietic precursor cells a method for inducing differentiation from pluripotent stem cells to hematopoietic stem cells and/or hematopoietic precursor cells by using the TGF-&beta inhibiter, and a cell culture product obtained by the differentiation inducing method.COPYRIGHT: (C)2012,JPO&INPIT【課題】多能性幹細胞、特にヒト多能性幹細胞から長期骨髄再構築能を持つ造血幹細胞を効率的に分化誘導し、増幅する手段を提供する。【解決手段】下記式等のトランスフォーミング増殖因子-β(TGF-β)阻害剤からなる、多能性幹細胞から造血幹細胞および/または造血前駆細胞への分化誘導促進剤、該TGF-β阻害剤を含む多能性幹細胞から造血幹細胞および/または造血前駆細胞への分化誘導促進用組成物、該TGF-β阻害剤を試薬として含む多能性幹細胞から造血幹細胞および/または造血前駆細胞への分化誘導促進用試薬キット、並びに該TGF-β阻害剤を使用することを特徴とする多能性幹細胞から造血幹細胞および/または造血前駆細胞への分化誘導方法、および該分化誘導方法により得られる細胞培養物。【選択図】なし
