An isolated, synthetic or recombinant nucleic acid comprising (a) a nucleic acid encoding at least one polypeptide, wherein the nucleic acid comprises a sequence having at least about 85%, 86%, 87%, 88%, 89% , 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity with SEQ ID NO: 1, and that has one or more changes of nucleotides encoding an amino acid change D61A or D61E, wherein the nucleic acid encodes at least one polypeptide having a hydrolase activity comprising palmitase activity, (b) a nucleic acid encoding at least one polypeptide having a hydrolase activity comprising palmitase activity, wherein the polypeptide comprises the sequence of SEQ ID NO: 2, or its enzymatically active fragments, and having at least one amino acid change D61A or D61E, (c) the nucleic acid of ( a) or (b) encoding a polypeptide having a hydrolase activity, which comprises palmitase activity but lacking a signal sequence, (d) the nucleic acid of any of (a) to (c) encoding a polypeptide that has a hydrolase activity comprising palmitase activity that further comprises a heterologous sequence, ( e) the nucleic acid of (d), wherein the heterologous sequence comprises, or consists of a sequence that encodes: (A) a heterologous signal sequence, (B) the sequence of (A), where the heterologous signal sequence derives of a heterologous enzyme, or, (C) a marker, an epitope, a targeting peptide, a cleavable sequence, a detectable moiety or an enzyme, or (f) a nucleic acid sequence completely complementary to the sequence of any of ( a) to (e).Un ácido nucleico aislado, sintético o recombinante que comprende (a) un ácido nucleico que codifica al menos un polipéptido, en donde el ácido nucleico comprende una secuencia que tiene al menos aproximadamente 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% o más identidad de secuencia con la SEQ ID NO: 1, y que tiene uno o más cambios de nucleótidos que codifican un cambio de aminoácido D61A o D61E, en dond
