Recombinant DNA derived from the genome of salmonid alphavirus, useful in vaccines for protection against this virus, includes a spacer between promoter and the cDNA prepared from viral RNA
Recombinant DNA (I) derived from the genome of a salmonid alphavirus (SAV) is new. It comprises (a) a transcriptional promoter and, downstream of this and under its control, (b) a spacer of at least 5 nucleotides (nt) and (c) the cDNA (Ia) from the genomic RNA of an SAV. Independent claims are included for: (1) preparing an RNA replicon of SAV by introducing (I) into a host cell and culturing the cell； (2) the RNA replicon prepared by method (1)； (3) method for preparing a recombinant SAV by introducing (I), or the replicon of (2), into a host cell where all structural proteins required for encapsidation are expressed, then growing the cells； (4) recombinant SAV prepared by method (3)； and (5) vaccine that contains (I), the replicon of (2) or recombinant SAV of (4). ACTIVITY : Antiviral. MECHANISM OF ACTION : Vaccine.Det beskrives rekombinante DNA'er omfattende cDNA av genomisk RNA av et Salmonidae a-virus som foregåes av en spacersekvens, under kontroll av en egnet promoter. Disse DNA'er er brukbare for å oppnå ekspresjonsvektorer, fremstilling av Salmonidae ?-virus og for å oppnå vaksiner.
