연세대학교 산학협력단; YONSEI UNIVERSITY;INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITY;INDUSTRY-ACADEMIC COOPERATION FOUNDATION
发明人:
LEE, Jin Woo,이진우,LEE, Seul Gi,이슬기,YOON, Dong Suk,윤동석
申请号：
KRKR2014/011329
公开号：
WO2015/088162A1
申请日:
2014.11.24
申请国别(地区):
KR
年份:
2015
代理人:
摘要：
The present invention relates to a composition for promoting differentiation from stem cells, more specifically mesenchymal stem cells to chondrocytes, a composition for prevention or treatment of osteochondral injury disease, and a method for screening the same. The present invention newly identified miR-495 as miRNA to inhibit differentiation from stem cells to chondrocytes and it was confirmed that pluripotent stem cells can be effectively differentiated to chondrocytes by the inhibition of miR-495. The present invention can be used usefully for essential prevention or treatment of diseases associated with osteochondral injury as well as osteoarthritis through the reproduction of the lost cartilage tissue.La présente invention concerne une composition permettant de favoriser la différenciation à partir des cellules souches, plus spécifiquement des cellules souches en chondrocytes, une composition pour la prévention ou le traitement des lésions ostéochondrales, et un procédé de dépistage associé. La présente invention a récemment identifié miR-495 comme le miARN inhibant la différenciation des cellules souches en chondrocytes et il a été confirmé que des cellules souches pluripotentes peuvent être efficacement différenciées en chondrocytes par l'inhibition de miR-495. La présente invention peut être utilisée à bon escient pour la prévention ou le traitement de base de maladies associées à une lésion ostéochondrale ainsi que de l'arthrose par le biais de la reproduction du tissu cartilagineux perdu.본 발명은 줄기세포, 보다 구체적으로는 중간엽 줄기세포의 연골세포로의 분화 촉진용 조성물, 연골 손상 질환의 예방 또는 치료용 조성물 및 이들의 스크리닝 방법에 관한 것이다. 본 발명은 줄기세포의 연골세포로의 분화를 저해하는 miRNA로서 miR-495를 새롭게 규명하였으며, 이의 저해를 통해 다능성을 가진 줄기세포를 연골세포로 효율적으로 분화시킬 수 있음을 확인하였다. 본 발명은 소실된 연골조직의 재생을 통해 퇴행성 관절염을 비롯한 연골손상 관련 질환의 근본적인 예방 및 치료에 유용하게 이용될 수 있다.
