The invention provides isolated Brevibacillius laterosporus strains with insecticidal activity against at least one Lepidopteraspecies and at least one Diptera species. In particular the invention provides the isolated B. laterosporus strains NMI No. V12/001946, NMI No. V12/001945 and NMI No. V12/001944. The invention provides compositions comprising one or more strain of the invention. The invention also provides methods of use of one or more strains or compositions of the inventions to control pests, particularly insect pests.