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Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops
专利权人:
E. I. DU PONT DE NEMOURS AND COMPANY
发明人:
BROGLIE KAREN E.,LIGHTNER JONATHAN EDWARD
申请号:
US20010743980
公开号:
US6570008(B1)
申请日:
2001.01.15
申请国别(地区):
美国
年份:
2003
代理人:
摘要:
The instant invention discloses utilization of a cDNA clone to construct sense and antisense genes for inhibition of starch synthase enzymatic activity in corn. More specifically, this invention concerns a method of controlling the starch fine structure of starch derived from the grain of cereal crops comprising: (1) preparing a chimeric gene comprising a nucleic acid fragment encoding a starch synthase structural gene or a fragment thereof, operably linked in either sense or antisense orientation on the upstream side to a nucleic acid fragment encoding a promoter that directs gene expression in corn endospern tissue, and operably linked on the downstream side to a nucleic acid fragment encoding a suitable regulatory sequence for transcriptional termination; and (2) transforming cereal crops with said chimeric gene, wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed cereal crops compared to the fine structure of starch derived from cereal crops not possessing said chimeric gene.
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