Rough mutants of Bacillus and methods of their application for accelerating the growth of plants, improving the health of plants and combating diseases and pests
ГИЛЯБЕР-ГОЙЯ Магали (US),ХОВИНГА Сара Ф. (US),ДЖУ Даниэл М. (US),МАРГОЛИС Джонатан С. (US),МИЛЛЗ Сара Й. (US),ТОМАС Варгиз (US),КЕРТИС Дамиан (US),РОЙАЛТИ Рид Нейт (US),УИТСОН Рой (US)
申请号：
RU2013133873/10
公开号：
RU2013133873A
申请日:
2011.12.19
申请国别(地区):
RU
年份:
2015
代理人:
摘要：
1. A composition comprising spore-forming bacterial cells with a mutation in the swrA ortholog that reduces the ability to swarm bacterial cells compared to isogenic bacterial cells without a mutation, and in which at least about 70% of the bacterial cells are spores. The composition according to claim 1, for which the ability to swarm is measured on the basis of growth on a non-liquid surface. The composition according to claim 1, in which the spore-forming bacterial cells with a mutation belong to the species Bacillus in the clade Bacillus subtilis. The composition according to claim 1, wherein the spore-forming bacterial cells with a mutation comprise at least 3.5% of all bacterial cells in the composition. The composition of claim 3, wherein the mutated spore-forming bacterial cells comprise a wild-type sfp ortholog. The composition of claim 5, wherein the mutated spore-forming bacterial cells further comprise a wild-type degQ ortholog and a wild-type epsC ortholog. The composition of claim 6, wherein each of the sfp ortholog, degQ ortholog, and epsC ortholog have at least about 90% sequence identity with the sfp gene, degQ gene, and epsC gene, respectively, from any of B. subtilis, B. amyloliquefaciens, B. pumilus, B. licheniformis, or B. atrophaeus. 8. The composition of claim 6, wherein at least about 90% sequence identity is present with the sfp gene, degQ gene, and epsC gene from any of B. subtilis strain 3610, B. amyloliquefaciens strain FZB42, B. pumilus strain SAFR-032, B licheniformis strain 14580 or B. atrophaeus strain 1942.9. The composition according to claim 3, in which the mutation additionally leads to the formation of a stronger biofilm compared with isogenic bacterial cells without mutation. The composition of claim 9, wherein the stronger biofilm is more1. Композиция, содержащая спорообразующие бактериальные клетки с мутацией в ортологе swrA, уменьшающей способность к роению бактериальных клеток по сравнению с изогенными бактериальными клеткам
