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УСОВЕРШЕНСТВОВАННЫЙ СПОСОБ ПОЛУЧЕНИЯ МИКРОГРАНУЛ
专利权人:
ДЕ РОГОЗИН ИНСТИТЬЮТ ИНК. (US)
发明人:
ГЭЗДА Лоуренс (US),ЛЭРЭМОР Мелисса (US),ХЭМИЛТОН Тимоти (US),СМИТ Бэрри (US)
申请号:
RU2014133367
公开号:
RU2014133367A
申请日:
2013.01.30
申请国别(地区):
RU
年份:
2016
代理人:
摘要:
1. A process for preparing a composition of matter comprising a sample of the cells in the pellet, containing agarose, wherein said granule is coated with agarose, comprising the following steps: (a) mixing the first sample of said cells with agarose to form a slurry (b) forming pellets from said slurry ( c) coating said pellets with a second solution of agarose, and (d) dispensing said granule coated sample of mineral oil, wherein said mineral oil sample maintained at a temperature gradient so that said granule ne forcibly relocated from mineral oil at a higher temperature in the mineral oil at a lower temperature.2. A method according to Claim. 1, wherein said secretory cells are kletki.3. A method according to Claim. 1, wherein said cells are cancer kletki.4. A method according to Claim. 1, wherein said cells are cancer stem kletki.5. A method according to Claim. 1, wherein said cells are islet kletki.6. A method according to Claim. 1, wherein said cells are stem kletki.7. A method according to claim. 6, wherein said cells are embryonic stem kletki.8. A method according to Claim. 1, wherein said cells are pluripotent kletki.9. A method according to Claim. 1 further comprising recovering beads from said suspension tool-funnel before coating said pellets with a second solution agarozy.10. A method according to Claim. 1 further comprising recovering said granules from said mineral oil-voronkoy.11 tool. A method according to Claim. 1, wherein said temperature gradient is from about 20 ° C to about 50 ° C, with higher temperature at the start gradienta.12. The method of claim. 11, where1. Способ получения композиции вещества, содержащей образец клеток в грануле, содержащей агарозу, где указанная гранула покрыта агарозой, включающий следующие стадии:(a) смешивание первого образца агарозы с указанными клетками с образованием суспензии(b) образование гранулы из указанной суспензии(c) покрытие указанной гранулы вторым раствором агарозы, и(d) дозирование указанной гранулы
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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