A method for quantifying a blood vessel reflection parameter associated with a biological subject, the method including, in at least one electronic processing device determining, from a fundus image of an eye of the subject, edge points of at least one blood vessel in a region near an optic disc, processing the fundus image, at least in part using the edge points, to identify blood vessel edges and central reflex edges, determining blood vessel and central reflex parameter values using the blood vessel edges and determining a blood vessel reflection parameter value at least partially indicative of blood vessel reflection using the blood vessel and central reflex parameter values.
