Soybeans (Glycine max) possessing a novel genetic allele for the production of reduced trypsin inhibitor in seeds is provided. Such alleles can be readily transferred to other soybean lines and cultivars. In a preferred embodiment a soybean plant possesses the combined presence of the Kunitz allele along with the SG-ULTI mutant allele, the combination of which was found to produce an ultra-low trypsin inhibitor phenotype in the resulting progeny seeds of a cross of a Kunitz allele into the 435.TCS background. A seed or seed product is made possible in this instance that is particularly well suited for consumption without extensive processing to remove trypsin inhibitor. The invention also relates to soybean seeds and plants containing the SG-ULTI mutant allele, and to methods for producing a soybean plant containing the SG-ULTI mutant allele produced by crossing a soybean plant containing the SG-ULTI mutant allele with itself or another soybean variety
