#$%^&*AU2010201787B220120913.pdf#####ABSTRACT A cultured comeal tissue construct includes a first layer of cultured corneal keratocyte cells cultured under conditions to produce a layer of extracellular matrix which is synthesized and assembled by the cultured comeal keratocyte cells, with the cultured comeal keratocyte cells contained within the synthesized extracellular matrix layer, wherein said extracellular matrix is produced by the cultured comeal keratocyte cells in the absence of exogenous matrix components or synthetic members during the culturing conditions； and , a second layer of cells comprising comeal epithelial cells disposed on the first layer. A method for producing the cultured comeal tissue is also described.
