The present invention can feed substrates without waste to generate seed sludge with high bacterial cell concentrations and can start up operation within a short time, in cultivating anaerobic ammonium-oxidizing bacteria with ammonium and nitrite as substrates. Equipment (10) for cultivating anaerobic ammonium-oxidizing bacteria, which cultivates, in a cultivation tank (12), novel anaerobic ammonium-oxidizing bacteria that anaerobically denitrify nitrite and ammonium used as substrates, comprises an ammonium feed device (14) which feeds ammonium at a given concentration into the cultivation tank (12), a nitrite feed device (16) which feeds nitrite at a given concentration into the cultivation tank (12), and a control device (22) which controls a feed rate Y of the substrate.
