MARQUES ERNESTO TORRES DE AZEVEDO,MACIEL FILHO ROMULO,DHALIA RAFAEL
申请号：
ZA201203862
公开号：
ZA201203862B
申请日:
2012.05.25
申请国别(地区):
ZA
年份:
2013
代理人:
摘要：
The present invention relates to DNA vaccines encoding specific viral sequences. DNA vaccines against yellow fever, developed according to the invention are based on the sequence encoding the envelope protein of VFA (p/YFE). In addition to wild construction p/YFE, the sequence E was also fused to the sequence encoding the protein Associated to the Lysosomal human Membrane (h-LAMP), generating the construction (pL/YFE). The results of the invention are considered very promising since the two constructions are capable of inducing T cell response against the same epitopes induced by the vaccine 17DD, and the construction pl/YFE was also capable of inducing neutralizing antibodies at a concentration considered satisfactory. The vector pL/YFE was also inoculated into mice for subsequent intracerebral challenge with VFA. Surprisingly, 100% of mice immunized with pL/YFE survived the challenge.
