A sutureless chest tube adhesion assembly configured to quickly and easily anchor a chest tube is described. The assembly generally comprises a notched flexible substrate adapted to adhere to human skin 415. A chest tube incision in a notched region of the substrate provides an entry point for the chest tube which is anchored to the substrate by way of an intermediate flexible membrane that covers and adheres to the chest tube and substrate. The intermediate flexible membrane has a matching notched region adapted to accommodate the chest tube. The chest tube and incision are adapted to be covered by a transparent cover sheet which covers and adheres to a portion of the substrate, the intermediate flexible membrane and to a portion of the human skin 415.