Methods of performing en face Doppler total retinal blood flow measurement are disclosed. The methods involve obtaining a set of structural OCT scans； calculating a noise-reduced set of Doppler phase shift images by dividing the full spectrum OCT fringe data into a set of narrower filtered frequency bands for phase shift calculation； removing bulk motion along a depth direction； detecting retinal vessels in the set of images； applying a phase unwrapping algorithm to the images； classifying the vessels as veins and arteries； and calculating total retinal blood flow based on that classification.
