An artificial chordae tendineae (100) and an artificial chordae tendineae implantation system are provided. The artificial chordae tendineae (100) includes a chordae tendineae main body (110). At least one end of the chordae tendineae main body (110) is connected to a fixing member (120). A side of the fixing member (120) facing away from the chordae tendineae main body (110) is provided with a puncturing connection member (125). The artificial chordae tendineae implantation system includes a clamping device (300), a puncturing device (400a)puncture needle, the artificial chordae tendineae (100), and a pushing device (200). The pushing device (200) includes a pushing shaft(210). The puncturing device (400a) and the clamping device (300) are received in the pushing shaft (210). The clamping device (300) includes a clamping push rod(330), a distal clamp (310) and a proximal clamp (320). The proximal clamp (320) is provided at a distal end of the pushing shaft (210), and the distal clamp (310) is provided at a distal end of the clamping push rod (330). The puncturing device (400a) includes a puncture needle (410). A distal end of the puncture needle (410) is provided with a tapered straight tip. The artificial chordae tendineae (100) is received in the clamping device (300). The fixing member (120) of the artificial chordae tendineae (100) corresponds to the puncture needle (410). A stable connection is formed between the artificial chordae tendineae (100) and the puncture needle (410), thereby saving surgery time.