A multitrophic, inland aquaculture system allows for the cultivation of aquatic animals as a food source and the cultivation of algae for use as biofuel and biomass in a sustainable manner based on the symbiotic nature of the organisms grown. This unique process minimizes biological, energy, and water inputs to create a highly sustainable system by utilizing the cyclic conversion of oxygen to carbon dioxide and back to oxygen as well as using the biological waste from aquatic food organisms to provide nutrients for algae organisms for the benefit of both species. This enables the cultivation of food species in higher densities than previously possible, and eliminates the need to provide additional nutrients, water, and non-atmospheric carbon dioxide for algal growth.