GOMEZ SEBASTIAN, Silvia,LOPEZ VIDAL, Javier,MARTINEZ ESCRIBANO, José Angel
申请号：
ES12750333
公开号：
ES2612854T3
申请日:
2012.06.12
申请国别(地区):
ES
年份:
2017
代理人:
摘要：
recombinant baculovirus containing Ac-ie-01 endogenous gene comprising [i] an additional cDNA copy of Ac-ie-01 baculovirus as a transgene under the control of a suitable promoter which allows expression above endogenous levels of the IE-1 protein, IE-0 and / or fragments thereof that function as transcription regulators, wherein the nucleic acid is selected from the group consisting of: a) a nucleic acid containing the nucleotide sequence indicated in any of SEQ ID NO: 1- 5 b) a nucleic acid having a sequence identity of at least 70%, preferably at least 75%, more preferably at least 80%, more preferably at least 85%, more preferably at least 90% and most preferably at least 95% to the nucleotide sequence shown in any of SEQ ID NO: 1-5 and encoding a protein that can function as a regulator of transcription in a recombinant baculovirus c) a nucleic acid encoding an amino acid containing the amino acid sequence shown in any of SEQ ID NO: 6-9 d) a nucleic acid sequence encoding an amino acid sequence having a sequence similarity of at least 70%, preferably at least 75%, more preferably at least 80%, more preferably at least 85%, more preferably at least 90% and most preferably at least 95% with amino acid sequence shown in any of SEQ ID NO: 6-9 and that may function as transcriptional regulator in a recombinant baculovirus [Ii] wherein the recombinant baculovirus further comprises at least one recombinant homologous region (hr) as region enhancer operably linked to any promoter that is suitable to drive expression of a recombinant protein, [iii] in which the promoter operably linked to the homologous region (hr) is selected from the group of nucleic acids comprising: a) a nucleic acid containing the nucleotide sequence shown in any of SEQ ID NO: 10-16 b) a nucleic acid sequence that can function as a promoter in a recombinant baculovirus which has a sequence identity of at least 70%, preferably at least 75%, more preferably at least 80%, more preferably at least 85%,
