AGRICULTURE AND AGRI-FOOD CANADA;BROWN, DANIEL, C., W.;BARRAN, LESLIE, R.;BROMFIELD, EDEN, S., P.
发明人:
BROWN, DANIEL, C., W.,BARRAN, LESLIE, R.,BROMFIELD, EDEN, S., P.
申请号：
WO1999CA00956
公开号：
WO0021358(A1)
申请日:
1999.10.13
申请国别(地区):
世界知识产权组织国际局
年份:
2000
代理人:
摘要：
Molecular and morphological data shows that sexual hybridization of Medicago laciniata M. sativa and Medicago laciniata Medicago truncatula was successful by using pre-flower opening emasculation, controlled pollination and pod/ovule/embryo culture techniques. The techniques are time consuming and inefficient at this point in time and result in a limited transfer of genetic material from the pollen donor. Further optimization of the techniques would likely improve efficiency. Recovered F1 hybrids consistently show morphological characteristics intermediate between the parents but characters are skewed toward the maternal parent. The morphological observations are supported by molecular evidence which shows that the majority of DNA in the hybrids in maternal in nature. The recovered F1 plants retained the plant nodulation specificity factor from M. laciniata and produced adequate amount of seed. This is the first report of a successful hybridization of these species. It is recommended that the F1 seed be tested in a plant breeding program for segregation of desirable agronomic characteristics, including nodulation specificity and crossing ability with alfalfa (M. sativa) at the diploid and tetraploid level. Crossing of hybrids with M. sativa may be difficult due to the predominance of M. laciniata genetic material. Further cycles of hybridization using the protocols described here may be necessary to increase the M. sativa germplasm component of the population to the point that it can be handled with conventional breeding approaches.
