A method for manufacturing a registration template for use in medical navigation system-guided surgery, comprising: producing a registration template having a surface, which precisely surface-bonds to a surface of a bone at a surgical target site of a patient, and having three or more registration points, from stereoscopic surface data ensuring precise surface bonding to the surface of the bone at the surgical target site of the patient, based on stereoscopic surface data created from tomography information on the bone of the surgical target site of the patient and providing a pedestal (4) for mounting optical trackingballs (7), which are used for three-dimensional position detection for a medical navigation system, at the center of the registration points. By using the template having curves surfaces which three-dimensionally precisely and intimately contact the bone surface of the patient, high-precision registration can be performed, and an operators man-hours immediately before surgery in an operating room can be reduced to shorten the surgery time.