HELMHOLTZ ZENTRUM MUENCHEN DEUTSCHES FORSCHUNGSZENTRUM FUER GESUNDHEIT UND UMWELT (GMBH)
发明人:
SCHEEL, CHRISTINA H.,LINNEMANN, JELENA R.,MEIXNER, LISA K.,MIURA, HARUKO
申请号：
CA2980852
公开号：
CA2980852A1
申请日:
2016.04.28
申请国别(地区):
CA
年份:
2016
代理人:
摘要：
The present invention is in the field of stem cell biology, in particular in the field of developmental and regenerative biology. The invention generally relates to a method of generating cells capable of differentiating to a multicellular organoid unit that morphologically and/or functionally resembles the terminal ductal-lobular unit. More precisely, said cells are generated by dissociating mammary epithelial tissue, thereby gaining cells and culturing said cells in presence of a compound which elevates cAMP levels in a collagen gel. Under said culturing conditions said cells form a multicellular organoid unit facilitating to obtain a breast stem cell by isolating a single cell from said multicellular organoid unit. The present invention also relates to enriching said cells and differentiating them to a multicellular organoid that morphologically and/or functionally resembles the terminal ductal-lobular unit and use of said cells or said multicellular organoid in testing a compound. Furthermore, the present invention relates to a composition comprising said breast stem cells or the multicellular organoid.
