IRARRAZABAL MUNOZ CARLOS ERNESTO,이라라자발 무노즈, 카를로스 에르네스토
申请号：
KR1020147009382
公开号：
KR1020140076571A
申请日:
2011.09.22
申请国别(地区):
KR
年份:
2014
代理人:
摘要：
The present invention relates to a method and a kit for monitoring, diagnosis, prognosis of acute kidney injury in early stage and determination of treatment in subjects suffering thereof. The method comprises the steps of a) providing a urine sample b) enriching the urine sample in exosomes present in the urine sample using at least one step of immunopurification c) detecting an acute kidney injury (AKI) marker in the exosome. The invention also relates to a kit for determining the presence and/or level of a specific kidney injury marker, for simple and early determination of the onset of AKI in a subject, the kit comprising means for enriching the urine sample in exosomes using at least one step of immunopurification, and means for detecting a predetermined kidney injury marker of a condition. The method and kit of the invention have the advantage to provide a specific, simple and early detection of kidney injury markers that is no possible with the standard methods applied until now. The method and kit of the invention also provide the advantage of identifying the specific structure that is injured in the kidney, since it uses specific antibodies for specific proteins present in specific structures of the kidney.본 발명은 급성 신장 손상을 조기에 모니터링, 진단, 예후하고, 그를 앓는 대상체에서 치료법을 결정하기 위한 방법 및 키트에 관한 것이다. 본 방법은 a) 요 샘플을 제공하는 단계 b) 하나 이상의 면역정제 단계를 이용하여 요 샘플에서 요 샘플에 존재하는 엑소좀을 농축시키는 단계 및 c) 엑소좀 중의 급성 신장 손상 (AKI) 마커를 검출하는 단계를 포함한다.
