AJITH ANAND,MAREN L. ARLING,ALEXANDRE DA SILVA CONCEICAO,WILLIAM JAMES GORDON-KAMM,CRAIG E. HASTINGS,GEORGE M. HOERSTER,THEODORE MITCHELL KLEIN,CARLOS M. LA ROTA,KEITH S. LOWE,SHIV BAHADUR TIWARI,NING
申请号：
US15249318
公开号：
US20170121722A1
申请日:
2016.08.26
申请国别(地区):
US
年份:
2017
代理人:
摘要：
The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains or (iii) a combination of (i) and (ii) and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
