#$%^&*AU2009202827A820120308.pdf#####ABSTRACT A first method of purifying apolipoprotein A-i includes mixing plasma fraction IV with a 1-8 M urea solution to form a pretreatment solution loading the pretreatment solution to a first anion chromatography column, and then eluting to obtain an apoA-1 protein solution and loading the apoA-1 protein solution to a second anion chromatography column, and eluting to obtain pure apoA-1 protein. A second method includes dissolving plasma fraction IV in a buffer to produce a pretreatment solution adding NaCl to the pretreatment solution and cooling it to form apoA-1 precipitate collecting and reconstituting the apoA-1 precipitate loading the reconstituted apoA-1 to an anion exchange column and eluting apoA-1 from the column. 17
