The present invention relates to a method for extracting matrine from Sophora flavescens by extracting Sophora flavescens under the temperature and pressure conditions of extracting a supercritical fluid. Favorably, the temperature condition is desirable to be 35-45C, but is not limited to the same. The pressure condition is 100.0-300.0 bar, favorably 300.0 bar, but is not limited to the same.본 발명은 고삼(Sophora flavescens)을 초임계유체 추출의 온도 및 압력 조건에서 추출하여 마트린을 추출하는 방법에 관한 것이다.