A detection method using a detection element, including a first electrode and a second electrode, in which a detection layer containing an enzyme is provided in the first electrode and the second electrode, includes a first step of measuring an electric current generated by decomposition of hydrogen peroxide generated from glucose in the detection layer of the first electrode as an electric current value between the first electrode and the second electrode, and a second step of measuring an electric current generated by decomposition of hydrogen peroxide generated from glucose in the detection layer of the second electrode as an electric current value between the first electrode and the second electrode.