An object of the present invention is to provide a novel microbial oil containing DGLQA at a high ratio. A specific amount of at least one polyunsaturated fatty acid having 20 or more carbon atoms in a fatty acid alkyl ester form and / or a free fatty acid form, and a carbon in a specific amount of a fatty acid alkyl ester form and / or a free fatty acid form A microbial oil containing a heat-generating fatty acid having a number of 16 to 22； a feedstock oil containing at least one polyunsaturated fatty acid having 20 or more carbon atoms in an alkyl ester form and / or a free fatty acid form obtained from the microbial biomass is prepared. A method for producing the above-mentioned raw material oil, which comprises performing precision distillation under specific conditions, and obtaining the above-mentioned microbial oil； a concentrated microbial oil obtained by using the above-mentioned production method and a method for producing the same； An agent for treating or preventing an inflammatory disease, comprising an oil or a concentrated microbial oil. [Selection diagram] None【課題】DGLQAなどを高比率で含む新規な微生物油の提供。【解決手段】特定量の脂肪酸アルキルエステル形態及び／又は遊離脂肪酸形態である少なくとも１の炭素数２０以上の多価不飽和脂肪酸と、特定量の脂肪酸アルキルエステル形態及び／又は遊離脂肪酸形態である炭素数１６～２２の熱生成脂肪酸とを含む微生物油；微生物バイオマスから得られたアルキルエステル形態及び／又は遊離脂肪酸形態の炭素数２０以上の少なくとも１つの多価不飽和脂肪酸を含む原料油を用意すること、前記原料油に対して、特定条件による精密蒸留を行うこと、前述の微生物油を得ることを含む当該製造方法；当該製造方法を利用して得られる濃縮微生物油及びその製造方法；当該微生物油又は濃縮微生物油を含む炎症性疾患治療又は予防剤。【選択図】なし
