於活體外進行抗體之糖基化工程农业专利-农业专业知识服务系统

发明人:: FALKENSTEIN, ROBERTO,佛肯斯丹　罗伯托,佛肯斯丹　羅伯托,WALCH, HEIKO,渥齐　赫科,渥齊　赫科,MALIK, SEBASTIAN,莫理克　赛巴斯钦,莫理克　賽巴斯欽,THOMANN, MARCO,索曼　马可,索曼　馬可,VON ROMAN, MATTHIAS FREIHERR,范　罗门　马谛斯　凡赫,范　羅門　馬諦斯　凡赫,SCHMID, INGRID,胥密特　英格,佛肯斯丹罗伯托,佛肯斯丹羅伯托,渥齐赫科,渥齊赫科,莫理克赛巴斯钦,莫理克賽巴斯欽,索曼马可,索曼馬可,范罗门马谛斯凡赫,范羅門馬諦斯凡赫,胥密特英格

摘要：: Herein is reported a method for producing an antibody comprising the steps of forming an antibody-antibody light chain affinity ligand complex, wherein the antibody light chain affinity ligand is immobilized on a solid phase, by applying a solution comprising the antibody to the immobilized antibody light chain affinity ligand, and incubating the complex formed in the previous step with one or more enzymes to modify the glycosylation of the antibody, thereby producing the antibody.本發明揭示一種用於產生抗體之方法，該方法包含以下步驟：形成抗體-抗體輕鏈親和配位體複合物，其中該抗體輕鏈親和配位體固定在固相上，藉由將包含該抗體之溶液施加至已固定之抗體輕鏈親和配位體，及將前一步驟所形成之複合物與一或多種酶一起培育，以修飾該抗體之糖基化，由此產生該抗體。