FRIEDMAN, JEFFREY,ZHANG, YIYING,PROENCA, RICARDO,MAFFEI, MARGHERITA,HALAAS, JEFFREY,GAJIWALA, KETAN,BURLEY, STEPHEN
申请号：
ES95929591
公开号：
ES2108663T3
申请日:
1995.08.17
申请国别(地区):
ES
年份:
2012
代理人:
摘要：
THE PRESENT INVENTION REFERENCES GENERALLY TO THE CONTROL OF BODY WEIGHT OF MAMMAL AND HUMAN ANIMALS, AND IN CONCRETE OF MATERIALS IDENTIFIED HEREBY AS WEIGHT MODULATORS, AND THE DIAGNOSTIC AND THERAPEUTIC USES FOR WHICH THERE ARE AVAILABLE MOD. IN ITS MOST EXTENSIVE ASPECT, THE PRESENT INVENTION CONCERNS THE EXTRACTION AND DISCOVERY OF NUCLEOTIDE SEQUENCES, AND PROTEINS EXPRESSED PUTATIVELY BY SUCH NUCLEOTIDES OR VARIATIONS DEGENERATED THEREOF, WHICH SHOW THE PART OF THE CAPACITY IN THE CAPACITY OF Mammals THE NUCLEOTIDE SEQUENCES IN QUESTION REPRESENT THE GENES CORRESPONDING TO THE OB GENE OF MURINE AND HUMAN, WHICH IT IS CONSIDERED TO PLAY A CRITICAL ROLE IN THE REGULATION OF WEIGHT AND BODY ADIPOSITY. PRELIMINARY DATA, PRESENTED HERE, SUGGEST THAT THE POLIPEPTIDE PRODUCT OF THE GEN IN QUESTION WORKS LIKE A HORMONE. THIS INVENTION PROVIDES ADDITION MOLECULES OF NUCLEIC ACID FOR USOCOMO MOLECULAR PROBES, OR AS INITIATORS FOR AMPLIFICATION OF CHAIN POLYMERASE (PCR) REACTION, THAT IS, SYNTHETIC OR NATURAL OLIGONUCLEOTIDES. IN OTHER ASPECTS, THE PRESENT INVENTION PROVIDES A CLONING VECTOR, WHICH UNDERSTANDS THE NUCLEIC ACIDS OF THE INVENTION； AND A BACTERIA, INSECT OR A MAMMAL EXPRESSION VECTOR, THAT INCLUDES THE NUCLEIC ACID MOLECULES OF THE INVENTION, OPERATIVELY ASSOCIATED WITH AN EXPRESSION CONTROL SEQUENCE. IN CONSEQUENCE, THE INVENTION FURTHER REFERS TO A BACTERIAL OR MAMMER CELL TRANSFERRED OR TRANSFORMED WITH AN APPROPRIATE EXPRESSION VECTOR, AND CORRESPONDINGLY, TO THE USE OF THE CONSTRUCTIONS ABOVE MENTIONED IN THE PREPARATION OF THE MODULATORS OF THE INVENTION. ANTIBODIES ARE ALSO PROVIDED FOR POLYPEPTIDE OB. IN ADDITION, A METHOD IS PROVIDED TO MODULATE THE BODY WEIGHT OF A MAMMER. IN SPECIFIC EXAMPLES, GENES ARE PROVIDED THAT CODIFY TWO ISOFORMS BOTH OF MURINE OR HUMAN POLYPEPTIDES.LA PRESENTE INVENCION SE REFIERE GENERALMENTE AL CONTROL DEL PESO CORPORAL DE ANIMALES INCLUIDOS MAMIFEROS Y HUMANOS, Y EN CONCRETO A MATERIALES IDENTIFICADOS AQUI COMO MODULA
