The present invention provides an anti-human TIM-3 antibody having high ADCC activity or antibody fragment thereof by screening a monoclonal antibody or antibody fragment thereof which binds to the amino acid sequence of the extracellular region of TIM-3 or its three-dimensional structure and exhibits ADCC activity； a hybridoma which produces the antibody； a DNA encoding the antibody； a vector comprising the DNA； a transformant which is obtainable by introducing the vector； a method for producing the antibody or the antibody fragment thereof which comprises using the hybridoma or the transformant； a therapeutic agent and a diagnostic agent comprising the antibody or the antibody fragment thereof as an active ingredient.
