ELSON, GREG,FOUQUE, NICOLAS,DEPOISIER, JEAN-FRANCOIS,FISCHER, NICOLAS,MAGISTRELLI, GIOVANNI
申请号：
CA2853011
公开号：
CA2853011A1
申请日:
2012.10.19
申请国别(地区):
CA
年份:
2013
代理人:
摘要：
The invention provides methods of purifying antibodies using various antibody- specific purification media to rapidly and efficiently separate mixtures of antibodies, antibody fragments and/or antibody components to isolate a desired antibody product from the mixture. The invention relates to the purification of bispecific monoclonal antibodies carrying a different specificity for each binding site of the immunoglobulin molecule, e.g., antibodies composed of a single heavy chain and two different light chains, one containing Kappa constant domain and the other a Lambda constant domain, including antibodies of different specificities that share a common heavy chain. The invention also provides the methods of efficiently purifying intact antibodies by separating the intact antibody from non-intact antibodies including free light chains.
