A device 2 for producing a three-dimensional structural body comprising an accommodating plate 3 in which an accommodating recess portion 3a for accommodating a cell mass 1 is formed, a support body 4 provided with a plurality of needle-shaped bodies 6 penetrating the cell mass, a suction nozzle 19 for adsorbing/holding the cell mass, and moving means 20 for moving the suction nozzle.The suction nozzle is provided with a tubular adsorbing portion 19a for adsorbing/holding one cell mass, and a diameter of the adsorbing portion is formed smaller than an outer diameter of the cell mass and larger than an outer peripheral diameter of a needle-shaped body 6a of the support body 4.When the cell mass is adsorbed/held by the adsorbing portion of the suction nozzle, the moving means moves the suction nozzle to above the needle-shaped body and further moves the suction nozzle in an axial direction of the needle-shaped body, the needle-shaped body is stuck into the cell mass, and the needle-shaped body penetrating the cell mass is inserted into the adsorbing portion.The three-dimensional structural cell can be produced automatically.