The present invention provides a method of cryopreserving a tissue derived from a pluripotent stem cell, including the following (1) to (3): (1) a first step of bringing a tissue derived from a pluripotent stem cell into contact with a cell protection solution that contains sulfoxide and chain polyol, (2) a second step of maintaining, in a cryopreservation solution, the pluripotent stem cell-derived tissue that was brought into contact with the cell protection solution in the first step, and, (3) a third step of cryopreserving, in the presence of a coolant, the pluripotent stem cell-derived tissue that was maintained in the cryopreservation solution in the second step. According to the method of the present invention, a stable preservation method of a tissue derived from a pluripotent stem cell can be provided.
