The invention discloses a probe and a method of manufacturing the same. The probe has a long cylindrical shape and includes a probe head(1), a probe body(2) at the rear of the probe head(1), and a probe tail(3) at a rear end of the probe body(2). The probe has a three-layer composite structure. The probe tail is a three-layer composite structure. The probe of the invention is used to detect a body tissue, and according to different electrical signals fed back by different body tissues, a type of the body tissue being detected by the probe can be known, thereby avoiding a medical accident in which a spinal cord or nerve is injured by a screw intruded into a vertebral foramen. The probe of the invention is simple in structure, convenient for use, easy in operation, of high reliability, high surgical safety and high success rate of surgery.