A method includes detecting a nucleotide sequence in genomic DNA obtained from a sample and evaluating a content of mesenchymal stem cells in the sample. The nucleotide sequence comprises at least one CpG site selected from the group consisting of: a CpG site consisting of C at position 157 and G at position 158 of SEQ ID NO: 1 and a CpG site complementary to the CpG site of SEQ ID NO: 1 in a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 1. The sample is an adipose tissue or a cell-containing fraction obtained from an adipose tissue.
