The disclosed invention provides a biofluid sensing device configured with a membrane-enhanced sensor located in a sweat collector. The disclosed analyte-specific sensor is configured to reduce required biofluid sample volume due to its close proximity to the skin and source of sweat biofluid. The sensor is contained within a pH and salinity-stabilized fluid that, in turn, is contained in a selectively permeable membrane to improve sensor performance in variable biofluids, and to protect the sensor from skin contact. In one embodiment, the biofluid sensing device includes means to protect the self-aligning sensors from abrasion against the skin or device components. Other embodiments of the disclosed invention include a track-etched membrane to provide sensor protection.
