Yunfeng Lin,Sirong Shi,Xiaoxiao Cai,Shiyu Lin,Qianshun LI
申请号：
US15555380
公开号：
US10507246B2
申请日:
2016.11.21
申请国别(地区):
US
年份:
2019
代理人:
摘要：
This invention discloses a method for preparing TDNs-AS1411-nucleic acid drug nanocomposite based drug delivery system, which includes the following steps: binding AS1411 and nucleic acid drug to a tetrahedral DNA nanostructure respectively； selecting four DNA single strands that respectively carry AS1411 and nucleic acid drug； mixing the four DNA single strands； mixing the DNA single strands and the TM buffer uniformly； putting the mixture into a PCR apparatus； raising the temperature to 95° C. quickly and maintaining for 10 min； and next cooling down to 4° C. and maintaining for 20 min to obtain the TDNs-AS1411-nucleic acid drug nanocomposite based drug delivery system. This drug delivery system can directly act on cell nucleus and will not be degraded by lysosomal. The targeting specificity is good. The drug can take a good efficacy and the pertinency is high.
