A decontamination system including a test fixture configured to engage an endoscope in order to retain the test fixture thereto, wherein the endoscope can comprise a valve chamber and a valve removable from the valve chamber such that the test fixture can be inserted therein. The test fixture can comprise, first, a frame having a first gripping portion and a valve member analog extending from the frame, and, second, a housing having a second gripping portion movable relative to the frame between a locked position and an unlocked position, wherein the second gripping portion is configured to be moved toward the first gripping portion to position the housing in its unlocked position. The second gripping portion can be configured to be moved away from the first gripping portion to position the housing in its locked position such that the housing can engage a locking feature on the endoscope.
